产品货号：
GS2080
中文名称：
Bse21I内切酶
英文名称：
Bse21I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Bse21I内切酶，Bse21I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CC↑TNAGG |
| 来源 | Bacillus species 21 |
| Assayed on | Lambda DNA(HindIII-digest) |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM KH2PO4(pH 7.4);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme about 50%of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10%PEG.Of these more than 90%can be recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 30 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AocI,AxyI,Bsu36I,CvnI,Eco81I,SauI |
相关搜索：Bse21I内切酶，Bse21I限制性内切酶